Chapter 1 Spectrophotomerty
1.1 Basic Concepts and Principle of Spectrophotometry
1.2 Measurement of light Absorption
1.3 Aplications of Spectrophotomerty
EXP.1 Quantitative Analysis of Protein
EXP.2 Determination of Blood Glucose
Chapter 2 Electrophoresis
2.1 Basic Principles of Electrophoresis
2.2 Impact Factors of Electrophoresis
2.3 Detection of Components after Electrophoreetic Separation
2.4 Special Electrophoreetic Techniques and Aplications
EXP.3 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis
EXP.4 Separation of Serum Lipoprotein by Agarose Gel Electrophoresis
EXP.5 Proteins Seperation by SDS-Polyacrylamide Gel Electrophoresis
Chapter 3 Chromatography
3.1 Fundamental Principles of Chromatography
3.2 Commonly Used Chromatographic Techniques
EXP.6 Detecting Transamination of Amino Acids by Paper Chromatography
EXP.7 Separation of Mixed Amino Acids by Cation Excharge Chromatography
EXP.8 Separation of Hemoglobin and Dinitrophrnyl-Glutsmste by Gel Filtration Chromatography
Chapter 4 Enzyme Analysis
4.1 The Activity of Enzyme
4.2 Enzyme Kinetics
4.3 Enzymatic analysis
EXP.9 Assay the Activity of ALT in Serum
EXP.10 LDH Analysis
EXP.11 Effect of Substacte Concentration on Enzyme Activity-Determining Km Value for Alkaline Phosphatase
EXP.12 Coupled enzymatatic raaction assay-Determination of Total Choleseterol,Triglycerides and Glucose in Serum
Chapter 5 Isolation and Purification of Protein
5.1 Methods and Basic Principles of Protein Purification
5.2 Procefure of Protein Preparation
5.3 Crystallization,concentration,Drying,and Storage of Protein
5.4 Identification and Analysis of Protein
EXP.13 Isolation and Identification of Serum IgG
EXP.14 Expression, Purificationand Identification of Glutatathione S-Transferase Fusion Protein
Chapter 6 Isolation, Purification and Identification of Nucleic Acids
6.1 Isolation, Purificationand of Nucleic Acids
6.2 Identification and analyusis of Nucleic Acids
EXP.15 Isolation of Eukaryotic Genomic DNA by Proteinase K-Phenol or NAI Method
EXP.16 SDS-Alkaline Lysis of Plasmid DNA
EXP.17 Isolation of Total RNA by TRIzol Reagent
EXP.18 Identification of DNA by UV-Sepctrophotomentry and Gle Electrophoresis
EXP.19 Identification of RNA by UV-Sepctrophotomentry and Formaladehyde Denaturating Agerose Gle Electrophoresis
EXP.20 Polymerase Chain Reaction and Reverse Transcription-Pcr
Chapter 7 Genetic Engineering
7.1 The Process of Gene Cloning
7.2 Enzymes for Gene Cloning
7.3 Vectors for Gene Cloning
7.4 Expression of Foreign Genes and Purification of Recombinant Protweins
EXP.21 DNA Cloning
EXP.22 Expression of Exogenous Gene in E.coli and Purification of the Expressional Product
English-Chinese Index